DNA Metabarcoding
Environmental DNA (eDNA) consists of genetic materials present in environmental samples such as soil, water, and sediment, derived from aquatic organisms and their traces, excretions, excrements, etc. DNA metabarcoding is the simultaneous barcoding of many organisms living in an environmental sample, using PCR amplification and high-throughput next-generation sequencing (NGS). It produces biodiversity data in the environment without relying on traditional sampling, culture, and taxonomy. It is also applied to predator feces that only contain tissue residuals of the prey and to verify the presence of endangered or rare species that are previously unreported. For successful analysis, it is important to use a short, versatile PCR primer set with a sufficiently high genetic variation that enables recovery and differentiation of all species within a taxonomic group. AquaGenTech provides DNA metabarcoding services for bacterial and eukaryotic communities and the taxonomic groups lowerthan the Eukaryota(e.g., fish and annelids) by designing taxon-specific PCR primer sets.
Analysis of DNA metabarcoding
1. Library Construction
Library Construction
  • eDNA extraction
  • 1st PCR amplification
  • 2nd PCR amplification
  • NGS analysis
2. Bioinformatic Analysis
Bioinformatic Analysis
  • Adaptor trimming
  • OTU clustering
  • Taxonomic assignment
  • Diversity indices