AquaAmp®
Fish BOL Universal PCR Kit
Fish BOL Universal PCR Kit
The AquaAmp® Fish BOL Universal PCR Kit was developed based on the patented technology of AquaGenTech Co., Ltd.
(Registration No. 10-2367357, Korean Intellectual Property Office). The kit consists of all PCR reagents (i.e., primers, DNA polymerase, dNTPs, buffer, etc.)
for fish DNA barcoding. Only the genomic DNA of the specimens needs to be prepared for the analysis using this kit.
The PCR primer set composed of each forward and reverse primer binds to the mitochondrial 16S ribosomal RNA gene (16S rDNA),
the alternative DNA barcoding region of animals. The primer set produced a single PCR band with an amplicon size of ca. 400 bp after
conventional PCR amplification. The primers were designed to be sufficiently conserved to anneal to nearly all fish species,
facilitating researchers to successfully produce clear single PCR bands. The kit can be easily used for DNA barcoding of both fresh fish and processed food specimens.
In addition, researchers conveniently performed DNA sequencing using specific adapters attached to the 5′ terminals of both forward and reverse primers.
The performance of AquaAmp® Fish BOL Universal PCR Kit was verified using 21 marine and freshwater fish specimens belonging
to diverse taxonomic groups (i.e., Pleuronectiformes, Perciformes, Gadiformes, Beloniformes, Synbranchiformes, Siluriformes, Osmeriformes, Anguilliformes,
Tetraodontiformes, Mugiliformes, Syngnathiformes, Scorpaeniformes, Lophiiformes, Salmoniformes, Chimaeriformes, Cypriniformes, Acipenseriformes, Clupeiformes,
Kurtiformes, and Gasterosteiformes). All specimens produced clear single PCR bands with the expected amplicon sizes.